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bioRxiv · 10.1101/2023.06.19.545558

Selective plane activation structured illumination microscopy

Temma, K.·Oketani, R.·Kubo, T.·Bando, K.·Maeda, S.·Sugiura, K.·Matsuda, T.·Heintzmann, R.·Kaminishi, T.·Fukuda, K.·Hamasaki, M.·Nagai, T.·Fujita, K.
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Abstract

Resolution enhancement in structured illumination microscopy is hindered when volumetric samples are observed owing to background signals from out-of-focus planes. In this study, we utilized selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. We demonstrated high-resolution fluorescence imaging of volumetric samples, including dense fluorescent objects in a cell spheroid.

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Temma, K., Oketani, R., Kubo, T., Bando, K., Maeda, S., Sugiura, K., Matsuda, T., Heintzmann, R., Kaminishi, T., Fukuda, K., Hamasaki, M., Nagai, T., Fujita, K.. 2023-06-19. Selective plane activation structured illumination microscopy. https://doi.org/10.1101/2023.06.19.545558

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