Goat PRP14 (gPRP14) has xeno-antigenic properties and works as a vaccine in preclinical models of cancer.

Minucci, S.; Bussolati, B.; Massa, P.; Brossa, A.; Ravasio, R.; Saadeldin, M.; Degani, G.; Papadimitriou, E.; Saadeldin, A.; Salvaggio, A.; Visentin, C.; Rizzi, G.; Ricagno, S.; Popolo, L.; Vanoni, M. A.; Pelicci, P. G.

doi:10.1101/2022.07.04.498594

bioRxiv · 10.1101/2022.07.04.498594

Goat PRP14 (gPRP14) has xeno-antigenic properties and works as a vaccine in preclinical models of cancer.

Minucci, S.·Bussolati, B.·Massa, P.·Brossa, A.·Ravasio, R.·Saadeldin, M.·Degani, G.·Papadimitriou, E.·Saadeldin, A.·Salvaggio, A.·Visentin, C.·Rizzi, G.·Ricagno, S.·Popolo, L.·Vanoni, M. A.·Pelicci, P. G.

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Abstract

We studied the activity of recombinant goat PRP14 (gPRP14), a member of the RID protein family, as a xeno-antigen in preclinical models of cancer. Antisera from rabbits and mice immunized with gPRP14 showed strong reactivity against several tumor cell types, which was absent towards normal cells: the tumor selectivity was related to surface and intra-cellular expression in tumor cells, and to an exclusively intra-cellular localization in normal cells. In vitro, binding to tumor cells was followed by cytotoxicity which could be rescued by the addition of excess soluble antigen. In vivo, an anti-tumor activity of immunization with gPRP14 was observed in murine syngeneic models of breast cancer and melanoma: the anti-tumor response was present when gPRP14 was administered in a preventive setting, and persisted upon repeated challenges with tumor cells in long-term survivor mice. Finally, we showed that both the humoral and T-cell mediated responses are needed for the optimal anti-tumor effect in the murine melanoma model. Thus, we have performed an initial characterization of gPRP14 as a cancer vaccine, which -given the potential wide range of tumor cells positive for the antigen-appears as a promising, novel immunotherapy.

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Minucci, S., Bussolati, B., Massa, P., Brossa, A., Ravasio, R., Saadeldin, M., Degani, G., Papadimitriou, E., Saadeldin, A., Salvaggio, A., Visentin, C., Rizzi, G., Ricagno, S., Popolo, L., Vanoni, M. A., Pelicci, P. G.. 2022-07-04. Goat PRP14 (gPRP14) has xeno-antigenic properties and works as a vaccine in preclinical models of cancer.. https://doi.org/10.1101/2022.07.04.498594

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