Biology subjects

Salvaggio, A.

Publications and source records attributed to Salvaggio, A..

5 recordsLinked to original sources

bioRxiv2025

A new skin supplement Purasomes XCELL: an integrative approach in regenerative aesthetics

Food supplements are taking on an increasingly important role as potential support tools for well-being and health. Supplements that offer essential nutrients and bioactive compounds can help fill nutritional gaps and provide specific benefits to complement health and wellness promotion strategies. Many supplements contain bioactive compounds used to enhance skin health and appearance, but only few biomolecules influence telomere lengths. Aims of this study was evaluated in vitro effectiveness of a new supplement Purasomes XCELL that contains natural compounds extracted from bovine colostrum and Astragalus, on skin aging using telomere length as an aging biomarker. Our results suggest that Purasomes XCELL has protective effect that is manifested already 4 weeks of treatment, but the telomere shortening rates on human fibroblasts were even more pronounced at 8 weeks. This new supplement could be highly effective on skin aging.

cell biology↗

bioRxiv2025

Innovations in skin aging: biomimetic exosomes versus natural exosomes

In this study, we compared the effectiveness of two commercial products contain biomimetic and natural exosomes on skin aging used two cells line, keratinocytes and fibroblasts. MTT assay and scratch-wound assay were employed to evaluate their in vitro effects. The treatment with product containing natural exosomes purified from bovine colostrum and loaded with growth factors and cytokines purified from bovine colostrum (AMPLEX PLUS technology), was able to significantly enhance cell proliferation of fibroblasts and keratinocytes at 24 hours compared to cells treatment with product containing biomimetic exosomes and compared to untreated cells (control). Product containing natural exosomes was also able to close the wound scratch after 24 hours. The obtained results suggest that the product containing exosomes derived from bovine colostrum has excellent structural and functional stability offer great potential as natural product for skin aging damage and wound healing.

cell biology↗

bioRxiv2025

A new product containing secretome purified from bovine colostrum, Purasomes IRC100+, in the treatment of vaginal atrophy: in vitro study to evaluate safety and effectiveness

Vaginal atrophy consists of the loss of elasticity and thinning of the vaginal tissues and mucous membranes. This is a condition that affects women especially during menopause, following the hormonal decline resulting from the cessation of ovarian activity. The objective of the present study was evaluated the effects of Purasomes IRC100+ on the proliferation and migration of VK2/E6E7 vaginal epithelial cells and on fibroblasts by in vitro experiments. We tested three different concentrations (0.5%, 1% and 2%). We found that cell viability was highest at 2% Purasomes IRC100+ concentration for both cell lines. The scratch-wound assay was also done. Compared with the cell scratch at 0 h, cells in all groups migrated after 24 h, the scratch was significantly reduced in treated cells compared to untreated cells. In particular, the cell migration rates were 77% and 82%, respectively for VK2/E6E7 cells and fibroblasts treated with 2% of Purasomes IRC100+. Finally, this product promotes vaginal epithelial cell proliferation and therefore it could improve vaginal atrophy and vaginal wound healing.

cell biology↗

bioRxiv2024

Exosomes for facial repair, regeneration and rejuvenation: comparison between different commercial products

Exosomes have been studied as a potential therapeutic option for improving skin texture. The mode of action of exosomes for skin texture likely involves several mechanisms, including stimulation of collagen production, promotion of skin cell proliferation; reduction of oxidative stress; regulation of inflammatory and antioxidant responses; extracellular matrix remodeling, which is important for maintaining skin structure and function. Aim of this study is to compare the in vitro effects of some commercial products that use new technologies containing plant derived nanovesicles from Rosa damascena callus, Centella asiatica callus, Euphorbia supina stem and exosomes from bovine colostrum passively loaded with growth factors and cytokines purified from bovine colostrum, for facial repair, regeneration and rejuvenation. Of all the products tested, only product containing exosomes purified from colostrum gave very encouraging results in terms of effects on proliferation, cell viability and wound repair. The other products tested gave results comparable to the untreated samples.

cell biology↗

bioRxiv2022

Goat PRP14 (gPRP14) has xeno-antigenic properties and works as a vaccine in preclinical models of cancer.

We studied the activity of recombinant goat PRP14 (gPRP14), a member of the RID protein family, as a xeno-antigen in preclinical models of cancer. Antisera from rabbits and mice immunized with gPRP14 showed strong reactivity against several tumor cell types, which was absent towards normal cells: the tumor selectivity was related to surface and intra-cellular expression in tumor cells, and to an exclusively intra-cellular localization in normal cells. In vitro, binding to tumor cells was followed by cytotoxicity which could be rescued by the addition of excess soluble antigen. In vivo, an anti-tumor activity of immunization with gPRP14 was observed in murine syngeneic models of breast cancer and melanoma: the anti-tumor response was present when gPRP14 was administered in a preventive setting, and persisted upon repeated challenges with tumor cells in long-term survivor mice. Finally, we showed that both the humoral and T-cell mediated responses are needed for the optimal anti-tumor effect in the murine melanoma model. Thus, we have performed an initial characterization of gPRP14 as a cancer vaccine, which -given the potential wide range of tumor cells positive for the antigen-appears as a promising, novel immunotherapy.

cancer biology↗