Biology subjects

Rivera, C.

Publications and source records attributed to Rivera, C..

4 recordsLinked to original sources

bioRxiv2018

Bumetanide prevents brain trauma-induced depressive-like behavior.

Brain trauma triggers a cascade of deleterious events leading to enhanced incidence of drug resistant epilepsies, depression and cognitive dysfunctions. The underlying mechanisms leading to these alterations are poorly understood and treatment that attenuates those sequels not available. Using controlled-cortical impact (CCI) as experimental model of brain trauma in adult mouse we found a strong suppressive effect of the sodium-potassium-chloride importer (NKCC1) specific antagonist bumetanide on appearance of depression-like behavior. We demonstrate that this alteration in behavior is associated with a block of CCI-induced decrease in parvalbumin-positive interneurons and impairment of post-traumatic secondary neurogenesis within the dentate gyrus of the hippocampus. The mechanism mediating the effect of bumetanide involves early transient changes in expression of chloride regulatory proteins and qualitative changes in GABA(A) mediated transmission after brain trauma. This work opens new perspectives in the early treatment of human post-traumatic induced depression. Our results strongly suggest that bumetanide might constitute an efficient prophylactic treatment to reduce neurological and psychiatric consequences of brain trauma.

neuroscience↗

bioRxiv2017

Prognostic biomarkers in oral squamous cell carcinoma: a systematic review

Over the years, several tumor biomarkers have been suggested to foresee the prognosis of oral squamous cell carcinoma (OSCC) patients. Here, we present a systematic review to identify, evaluate and summarize the evidence for OSCC reported markers. Eligible studies were identified through a literature search of MEDLINE/PubMed until January 2016. We included primary articles reporting overall survival, disease-free survival and cause-specific survival as outcomes. Our findings were analysed using REporting recommendations for tumor MARKer prognostic studies (REMARK), QuickGo tool and SciCurve trends. We found 41 biomarkers, mostly proteins evaluated by immunohistochemistry. The selected studies are of good quality, although, any study referred to a sample size determination. Considering the lack of follow-up studies, the molecules are still potential biomarkers. Further research is required to validate these biomarkers in well-designed clinical cohort-based studies.

pathology↗

bioRxiv2017

Oral diseases: a 14-year experience of a Chilean institution with a systematic review from eight countries

BACKGROUNDRetrospective studies to assess the distribution of oral diseases (ODs) are helpful in estimating the prevalence of oral diagnoses in the population, and thus help in preventive and curative services. Prevalence and frequency data for ODs are available from many countries, but information from Chile is scarce.\n\nMETHODSThis study investigated the frequency of ODs in a Chilean population. For this, we included all patients treated at the University of Talca (UTALCA, Chile) between 2001 and 2014. Patient characteristics were retrieved from medical files. To contextualize our results, we conducted a systematic review (SystRev) using Publish or Perish software (PoP), Google Scholar and MEDLINE/PubMed.\n\nRESULTSOne hundred sixty-six ODs were diagnosed, and the most prevalent groups were soft tissue tumours, epithelial pathology and salivary gland pathology. Individually, irritation fibroma, oral lichen planus (OLP) and mucocele were the most common diagnoses. ODs frequently affected unspecified parts of the mouth (including cheek, vestibule and retromolar area), gum, lips, tongue and palate. In the SystRev, the more studied diagnoses were leukoplakia, OLP and recurrent aphthous stomatitis; prevalent lesions included Fordyces spots, recurrent aphthous stomatitis and fissured tongue. Chilean patients and SistRev shared almost all ODs.\n\nCONCLUSIONThe results reflect ODs diagnosed in a specialized service of oral pathology and medicine in Chile and will allow the establishment of preventive/curative policies, adequate health services and dentistry curriculum.

pathology↗

bioRxiv2017

Agarose Floor Technique: A Simple Scaffold-Free Method For 3D Cell Culture And Multicellular Tumor Spheroids Formation

To the editors To the editors REFERENCES 3D cell cultures can close the gap between in vitro experiments used for discovery and in vivo designs used for efficacy and safety assessment before proceeding to clinical studies 1. 3D multicellular tumor spheroids reflect better the tumor environment in terms of phenotypic heterogeneity, nutrient and oxygen gradients, intervascular domains, and micrometastases 2.\n\nThis letter presents the agarose floor technique to obtain single-cell suspensions from oral cancer cells in nonadherent conditions. SCC-9 cells (American Type Culture Collection, Manassas, VA, USA) were cultured in culture plastic wares with nonadhesive surface. 150 mm dish are made of nonadhesive for cells by coating with 3.2% sterile agarose (Analytical Grade, Promega, Madison, WI, USA) thin films (5-8 mL/dish). This ...

cancer biology↗