Multi-scale electrophysiological characterization of human cardioids reveals progressive maturation

Vashanthakumar, V. K.; Naveed, M.; Wisnumurti, E.; Alerni, N.; Conrad, L.; Tissieres, V.; Tuchler, J.; Barozzi, I.; Ordog, B.; Horvath, A.; Osterwalder, M.; Odening, K. E.

doi:10.64898/2026.09.29.755336

bioRxiv · 10.64898/2026.09.29.755336

Multi-scale electrophysiological characterization of human cardioids reveals progressive maturation

Vashanthakumar, V. K.·Naveed, M.·Wisnumurti, E.·Alerni, N.·Conrad, L.·Tissieres, V.·Tuchler, J.·Barozzi, I.·Ordog, B.·Horvath, A.·Osterwalder, M.·Odening, K. E.

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Abstract

Background: Human induced pluripotent stem cell-derived cardiomyocytes are valuable for cardiac disease modelling and drug testing but remain limited by immature structural and electrophysiological properties in conventional two-dimensional cultures. Three-dimensional cardiac models, including cardioids, provide a more physiological environment through multicellular organization and enable tissue-level functional analysis. Their electrophysiological properties and maturation, however, remain incompletely characterized. Methods: We performed an integrated electrophysiological characterization of human cardioids over the first three weeks of maturation using sharp microelectrode recordings, calcium transient measurements, whole-cell patch clamp recordings, immunofluorescence, transcriptomic analysis, and pharmacological interventions. Results: During maturation, cardioids exhibited progressive electrophysiological maturation, characterized by more physiological resting membrane potentials (-54.0{+/-}3.1mV to -68.1{+/-}1.2mV), increased action potential amplitude (73.5{+/-}4.8mV to 101.7{+/-}2.1mV) and upstroke velocity (44.3{+/-}5mV/ms to 86.6{+/-}8.4mV/ms), and shorter action potential duration (178.9{+/-}8.7ms to 144.0{+/-}4.8ms at 1Hz stimulation). These changes were associated with progressively increased INa density and enhanced SCN5A mRNA and Nav1.5 protein expression, consistent with progressive maturation of cardiac excitability. IKr current and KCNH2/hERG expression were already present at early maturation stages and remained stable, as evidenced by preserved sensitivity to dofetilide-blockade. Pharmacological inhibition of barium- and HMR-sensitive currents altered resting membrane and/or action potential characteristics in intact cardioids, whereas in isolated cardiomyocytes classical IK1 remained undetectable and IKs was small, highlighting a certain degree of persisting immaturity of the model. Conclusions: These findings demonstrate that human cardioids undergo progressive electrophysiological maturation and provide complementary tissue- and cellular-level insights into cardiac function, establishing their suitability for investigating INa and IKr-related cardiac pathologies.

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Vashanthakumar, V. K., Naveed, M., Wisnumurti, E., Alerni, N., Conrad, L., Tissieres, V., Tuchler, J., Barozzi, I., Ordog, B., Horvath, A., Osterwalder, M., Odening, K. E.. 2026-10-05. Multi-scale electrophysiological characterization of human cardioids reveals progressive maturation. https://doi.org/10.64898/2026.09.29.755336

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