Multi-dimensional diffusion MRI at ultra-high gradient strength for mapping axonal architecture and microstructure in the primate brain

Gong, T.; Maffei, C.; Sung, D.; Bell, E.; Wu, J.; Shao, J.; Rosenblum, E. W.; Zeng, X.; Ramos-Llorden, G.; Müller, A.; Mahmutovic, M.; Keil, B.; Gunalan, K.; Ghosh, S.; Augustinack, J. C.; Huang, S. Y.; Haber, S. N.; Yendiki, A.

doi:10.64898/2026.02.18.705310

bioRxiv · 10.64898/2026.02.18.705310

Multi-dimensional diffusion MRI at ultra-high gradient strength for mapping axonal architecture and microstructure in the primate brain

Gong, T.·Maffei, C.·Sung, D.·Bell, E.·Wu, J.·Shao, J.·Rosenblum, E. W.·Zeng, X.·Ramos-Llorden, G.·Müller, A.·Mahmutovic, M.·Keil, B.·Gunalan, K.·Ghosh, S.·Augustinack, J. C.·Huang, S. Y.·Haber, S. N.·Yendiki, A.

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Abstract

We present the most comprehensive sampling of the macaque and human brain with diffusion MRI to date. As part of the BRAIN CONNECTS center for Large-scale Imaging of Neural Circuits, we leverage ultra-high-gradient MRI systems, including the first-of-its-kind Connectome 2.0, for post-mortem acquisitions. Each sample is imaged for [~]250 hours at multiple spatial resolutions down to 0.25 mm isotropic for whole macaque brains and 0.4 mm isotropic for human hemispheres. Our optimized protocols allow us to sample both species across [~]50 diffusion shells varying in b-value, diffusion time, and echo time, reaching ultra-high b-values up to 64000 s/mm2 with high signal-to-noise ratio. We demonstrate that these multi-dimensional data resolve not only white matter connectional architecture but also cortical and subcortical cytoarchitectonic boundaries, at a level of detail previously inaccessible in whole-brain noninvasive imaging. As such, these data are an important resource for both technical development and basic and clinical neuroscience.

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Gong, T., Maffei, C., Sung, D., Bell, E., Wu, J., Shao, J., Rosenblum, E. W., Zeng, X., Ramos-Llorden, G., Müller, A., Mahmutovic, M., Keil, B., Gunalan, K., Ghosh, S., Augustinack, J. C., Huang, S. Y., Haber, S. N., Yendiki, A.. 2026-02-18. Multi-dimensional diffusion MRI at ultra-high gradient strength for mapping axonal architecture and microstructure in the primate brain. https://doi.org/10.64898/2026.02.18.705310

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