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bioRxiv · 10.1101/635995

Cell Generation Dynamics Underlying Naïve T Cell Homeostasis in Adult Humans

Abstract

Thymic involution and proliferation of naive T cells both contribute to shaping the naive T cell repertoire as humans age, but a clear understanding of the roles of each throughout a human lifespan has been difficult to determine. By measuring nuclear bomb test-derived 14C in genomic DNA we determined the turnover rates of CD4+ and CD8+ naive T cell populations and defined their dynamics in healthy individuals ranging from 20-65 years of age. We demonstrate that naive T cell generation decreases with age, and that this could be explained by a combination of declining cell loss, peripheral division and thymic production during adulthood. We investigated putative mechanisms underlying age-related changes in homeostatic regulation of naive T cell turnover using mass cytometry to profile candidate signaling pathways involved in T cell activation and proliferation in CD4+ naive T cells relative to CD31 expression, a marker of thymic proximity. We show that basal NF-{kappa}B phosphorylation inversely correlated with CD31 expression and thus is decreased in peripherally expanded naive T cell clones. Functionally we found that NF-{kappa}B signaling was essential for naive T cell proliferation to the homeostatic growth factor IL-7, and reduced NF-{kappa}B phosphorylation in CD4+CD31- naive T cells is linked to reduced homeostatic proliferation potential. Our results reveal an age-related decline in naive T cell turnover as a putative regulator of naive T cell diversity and identify a molecular pathway that restricts proliferation of peripherally expanded naive T cell clones that accumulate with age.

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BibTeXRIS

Mold, J., Reu, P., Olin, A., Bernard, S., Michaëlsson, J., Rane, S., Yates, A., Khosravi, A., Salehpour, M., Possnert, G., Brodin, P., Frisen, J.. 2019-05-13. Cell Generation Dynamics Underlying Naïve T Cell Homeostasis in Adult Humans. https://doi.org/10.1101/635995

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