Generation of Temperature Sensitive Mutations with Error-Prone PCR in a Gene Encoding a Component of the Spindle Pole Body in Fission Yeast

Tang, N. H.; Fong, C. S.; Masuda, H.; Jourdain, I.; Yukawa, M.; Toda, T.

doi:10.1101/595447

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bioRxiv · 10.1101/595447

Generation of Temperature Sensitive Mutations with Error-Prone PCR in a Gene Encoding a Component of the Spindle Pole Body in Fission Yeast

Tang, N. H.·Fong, C. S.·Masuda, H.·Jourdain, I.·Yukawa, M.·Toda, T.

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Abstract

Temperature-sensitive (ts) mutants provide powerful tools, thereby investigating cellular functions of essential genes. We report here a simple procedure to generate ts mutations using error-prone PCR in pcp1 that encodes a spindle pole body (SPB) component in Schizosaccharomyces pombe. This manipulation is not restricted to analysis of Pcp1, and can be suited to any essential genes involved in other processes.

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Tang, N. H., Fong, C. S., Masuda, H., Jourdain, I., Yukawa, M., Toda, T.. 2019-04-01. Generation of Temperature Sensitive Mutations with Error-Prone PCR in a Gene Encoding a Component of the Spindle Pole Body in Fission Yeast. https://doi.org/10.1101/595447

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