Generation of Temperature Sensitive Mutations with Error-Prone PCR in a Gene Encoding a Component of the Spindle Pole Body in Fission Yeast
Temperature-sensitive (ts) mutants provide powerful tools, thereby investigating cellular functions of essential genes. We report here a simple procedure to generate ts mutations using error-prone PCR in pcp1 that encodes a spindle pole body (SPB) component in Schizosaccharomyces pombe. This manipulation is not restricted to analysis of Pcp1, and can be suited to any essential genes involved in other processes.
genetics↗