Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

Glaser, A. K.; Reder, N. P.; Chen, Y.; Yin, C.; Wei, L.; Kang, S.; Barner, L. A.; Xie, W.; McCarty, E. F.; Mao, C.; Halpern, A. R.; Stoltzfus, C. R.; Daniels, J. S.; Gerner, M. Y.; Nicovich, P. R.; Vaughan, J. C.; True, L. D.; Liu, J. T. C.

doi:10.1101/548107

bioRxiv · 10.1101/548107

Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues

Glaser, A. K.·Reder, N. P.·Chen, Y.·Yin, C.·Wei, L.·Kang, S.·Barner, L. A.·Xie, W.·McCarty, E. F.·Mao, C.·Halpern, A. R.·Stoltzfus, C. R.·Daniels, J. S.·Gerner, M. Y.·Nicovich, P. R.·Vaughan, J. C.·True, L. D.·Liu, J. T. C.

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Abstract

Recent advances in optical clearing and light-sheet microscopy have provided unprecedented access to structural and molecular information from intact tissues. However, current light-sheet microscopes have imposed constraints on the size, shape, number of specimens, and compatibility with various clearing protocols. Here we present a multi-immersion open-top light-sheet microscope that enables simple mounting of multiple specimens processed with a variety of protocols, which will facilitate wider adoption by preclinical researchers and clinical laboratories. One Sentence SummaryGlaser et al. describe a multi-immersion open-top light-sheet microscope that enables simple and high-throughput imaging of large numbers of preclinical and clinical specimens prepared with a variety of clearing protocols.

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Glaser, A. K., Reder, N. P., Chen, Y., Yin, C., Wei, L., Kang, S., Barner, L. A., Xie, W., McCarty, E. F., Mao, C., Halpern, A. R., Stoltzfus, C. R., Daniels, J. S., Gerner, M. Y., Nicovich, P. R., Vaughan, J. C., True, L. D., Liu, J. T. C.. 2019-02-13. Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues. https://doi.org/10.1101/548107

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