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bioRxiv · 10.1101/520429

Calcium-driven regulation of voltage-sensing domains in BK channels

Latorre, R.·Lorenzo-Ceballos, Y.·Carrasquel-Ursulaez, W.·Castillo, K.·Alvarez, O.
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Abstract

Allosteric interplays between voltage-sensing domains (VSD), Ca2+-binding sites, and the pore domain govern the Ca2+- and voltage-activated K+ (BK) channel opening. However, the functional relevance of the Ca2+- and voltage-sensing mechanisms crosstalk on BK channel gating is still debated. We examined the energetic interaction between Ca2+ binding and VSD activation measuring and analyzing the effects of internal Ca2+ on BK channels gating currents. Our results indicate that the Ca2+ sensors occupancy has a strong impact on the VSD activation through a coordinated interaction mechanism in which Ca2+ binding to a single -subunit affects all VSDs equally. Moreover, the two distinct high-affinity Ca2+-binding sites contained in the C-terminus domains, RCK1 and RCK2, appear to contribute equally to decrease the free energy necessary to activate the VSD. We conclude that voltage-dependent gating and pore opening in BK channels is modulated to a great extent by the interaction between Ca2+ sensors and VSDs.

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Latorre, R., Lorenzo-Ceballos, Y., Carrasquel-Ursulaez, W., Castillo, K., Alvarez, O.. 2019-01-16. Calcium-driven regulation of voltage-sensing domains in BK channels. https://doi.org/10.1101/520429

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