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bioRxiv · 10.1101/435461

Epigenetic transmission of cardiometabolic risk in offspring discordant for maternal gestational metabolic fitness

Guenard, F.·Marceau, S.·Simard, S.·Kral, J. G.·Vohl, M.-C.·Marceau, P.
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Abstract

OBJECTIVEDiabesity during gestation predisposes offspring to lifetime cardiometabolic risk. We demonstrated that maternal biliopancreatic diversion surgery (BPD) improved metabolic fitness and pregnancies reducing cardiometabolic risk in siblings born after (AMS) compared to before maternal surgery (BMS). We found differential methylation of inflammatory and glucoregulatory genes in peripheral blood cell DNA in offspring and in mothers after BPD compared to preoperative \"control\" women. Here we study offspring trajectories of cardiometabolic risk markers and determine persistence of the methylome related to body weight (BMI) and gestational weight gain (GWG).\n\nRESEARCH DESIGN AND METHODSProspective, cross-sectional study of 133 mothers with 89 BMS and 183 AMS offspring born mean 4 years after BPD and 83 unoperated control women was conducted, and differential methylation patterns in mothers were compared with those of offspring during 2-26 years.\n\nRESULTSIndependent of maternal or offspring BMI and GWG, postoperative maternal metabolic fitness was associated with improved cardiometabolic phenotype in AMS vs. BMS offspring sustained beyond puberty. BMS offspring exhibited increasing linear trajectories of weight, cardiometabolic and inflammation risk factors versus normative horizontal trajectories of AMS offspring. Methylation differences between AMS and BMS offspring identified 45 625 differentially methylated sites, 73% overlapping with those of mothers vs. controls; 4 446 demonstrated similar sustained directionality of differences in methylation levels; 154 sites exhibited significant correlation coefficients (r[≥]0.4) overrepresented within genes associated with cardiometabolic risk, growth and inflammation.\n\nCONCLUSIONMaternal BPD appears to epigenetically prevent transmission of cardiometabolic risk independent of BMI.

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Guenard, F., Marceau, S., Simard, S., Kral, J. G., Vohl, M.-C., Marceau, P.. 2018-10-04. Epigenetic transmission of cardiometabolic risk in offspring discordant for maternal gestational metabolic fitness. https://doi.org/10.1101/435461

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