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bioRxiv · 10.1101/412858

Heterogeneity of Sonic Hedgehog Response Dynamics and Fate Specification in Single Neural Progenitors

Xiong, F.·Tentner, A. R.·Hiscock, T. W.·Huang, P.·Megason, S.
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Abstract

During neural tube patterning, a gradient of Sonic hedgehog (Shh) signaling specifies ventral progenitor fates. The cellular response to Shh is processed through a genetic regulatory network (GRN) to specify distinct fate decisions. This process integrates Shh response level, duration and other inputs and is affected by noise in signaling and cell position. How reliably Shh response profile predicts the fate choice of a single cell is unclear. Here we use live imaging to track neural progenitors in developing zebrafish and quantify both Shh and fate reporters in single cells over time. We found that there is significant heterogeneity between Shh response and fate choice in single cells. We quantitatively modeled reporter intensities to obtain single cell response levels over time and systematically evaluated their correlation with cell fate specification. Motor neuron progenitors (pMN) exhibit a high degree of variability in their Shh responses, which is particularly prominent in the posterior neural tube where the Shh response dynamics are similar to those of the more ventrally fated lateral floor plate cells (LFPs). Our results highlight the precision limit of morphogen-interpretation GRNs in small and dynamic target cell fields.

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Xiong, F., Tentner, A. R., Hiscock, T. W., Huang, P., Megason, S.. 2018-09-10. Heterogeneity of Sonic Hedgehog Response Dynamics and Fate Specification in Single Neural Progenitors. https://doi.org/10.1101/412858

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