Multiple infection of cells changes the dynamics of basic viral evolutionary processes

Abstract

The infection of cells by multiple copies of a given virus can impact virus evolution in a variety of ways, for example through recombination and reassortment, or through intra-cellular interactions among the viruses in a cell, such as complementation or interference. Surprisingly, multiple infection of cells can also influence some of the most basic evolutionary processes, which has not been studied so far. Here, we use computational models to explore how infection multiplicity affects the fixation probability of mutants, the rate of mutant generation, and the timing of mutant invasion. This is investigated for neutral, disadvantageous, and advantageous mutants. Among the results, we note surprising growth dynamics for neutral and disadvantageous mutants: Starting from a single mutant-infected cell, an initial growth phase is observed which is more characteristic of an advantageous mutant and is not observed in the absence of multiple infection. Therefore, in the short term, multiple infection increases the chances that neutral or dis-advantageous mutants are present. Following this initial growth phase, however, the mutant dynamics enter a second phase that is driven by neutral drift or negative selection, respectively, which determines the long-term fixation probability of the mutant. Contrary to the short-term dynamics, the probability of mutant fixation, and thus existence, is lower in the presence compared to the absence of multiple infection, and declines with infection multiplicity. Hence, while infection multiplicity promotes mutant existence in the short term, it makes it less likely in the longer term. Understanding of these dynamics is essential for the investigation of more complex viral evolutionary processes, for which the dynamics described here for the basis. We demonstrate relevance to the interpretation of experiments in the context of published data on phage {varphi}6 evolution at low and high multiplicities.