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bioRxiv · 10.1101/339663

Tandem repeats drive variation of intrinsically disordered regions in budding yeast

Babokhov, M.·Reinfeld, B. I.·Hackbarth, K.·Bentov, Y.·Fuchs, S. M.
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Abstract

Copy-number variation in tandem repeat coding regions is more prevalent in eukaryotic genomes than current literature suggests. We have reexamined the genomes of nearly 100 yeast strains looking to map regions of repeat variation. From this analysis we have identified that length variation is highly correlated to intrinsically disordered regions (IDRs). Furthermore, the majority of length variation is associated with tandem repeats. These repetitive regions are rich in homopolymeric amino acid sequences but nearly half of the variation comes from longer-repeating motifs. Comparisons of repeat copy number and sequence between strains of budding yeast as well as closely related fungi suggest selection for and conservation of IDR-related tandem repeats. In some instances, repeat variation has been demonstrated to mediate binding affinity, aggregation, and protein stability. With this analysis, we can identify proteins for which repeat variation may play conserved roles in modulating protein function.

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Babokhov, M., Reinfeld, B. I., Hackbarth, K., Bentov, Y., Fuchs, S. M.. 2018-06-05. Tandem repeats drive variation of intrinsically disordered regions in budding yeast. https://doi.org/10.1101/339663

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