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bioRxiv · 10.1101/263129

No unexpected CRISPR-Cas9 off-target activity revealed by trio sequencing of gene-edited mice

Iyer, V.·Boroviak, K.·Thomas, M.·Doe, B.·Ryder, E.·Adams, D.
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Abstract

Introduction Introduction Results and Discussion Conclusion Author Contributions Competing Financial Interests 1. gRNA choices and... 2. Zygote injection 3. DNA extraction from... 4. Amplicon sequencing and... 5. Whole Genome Sequencing 6. Probability of detecting... 7. Variant calling, Trio... 8. Intersection of all... 9. Comparison of SNVs... 10. Validation with PCR... 11. Re-analysis of De... Supplementary Figure Supplementary tables References CRISPR-Cas technologies have transformed genome-editing of experimental organisms and have ...

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Iyer, V., Boroviak, K., Thomas, M., Doe, B., Ryder, E., Adams, D.. 2018-02-09. No unexpected CRISPR-Cas9 off-target activity revealed by trio sequencing of gene-edited mice. https://doi.org/10.1101/263129

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