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bioRxiv · 10.1101/243030

Crumble: reference free lossy compression of sequence quality values

Bonfield, J. K.·Durbin, R.·McCarthy, S. A.
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Abstract

MotivationThe bulk of space taken up by NGS sequencing CRAM files consists of per-base quality values. Most of these are unnecessary for variant calling, offering an opportunity for space saving.\n\nResultsOn the CHM1+CHM13 test set, a 17 fold reduction in quality storage can be achieved while maintaining variant calling accuracy.\n\nAvailabilityCrumble is OpenSource and can be obtained from https://github.com/jkbonfield/crumble.\n\nContactjkb@sanger.ac.uk\n\nSupplementary informationSupplementary data are available.

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Bonfield, J. K., Durbin, R., McCarthy, S. A.. 2018-01-05. Crumble: reference free lossy compression of sequence quality values. https://doi.org/10.1101/243030

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