Crumble: reference free lossy compression of sequence quality values
MotivationThe bulk of space taken up by NGS sequencing CRAM files consists of per-base quality values. Most of these are unnecessary for variant calling, offering an opportunity for space saving.\n\nResultsOn the CHM1+CHM13 test set, a 17 fold reduction in quality storage can be achieved while maintaining variant calling accuracy.\n\nAvailabilityCrumble is OpenSource and can be obtained from https://github.com/jkbonfield/crumble.\n\nContactjkb@sanger.ac.uk\n\nSupplementary informationSupplementary data are available.
bioinformatics↗