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bioRxiv · 10.1101/2025.10.16.682632

Systemic induced plant resistance genes expression in winter wheat under treatment with antagonistic bacteria of Fusarium sp.

Mekhantseva, K. V.·Vasilchenko, N.·Prazdnova, E.·Usatov, A.·Mazanko, M.·Chistyakov, V.
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Abstract

IntroductionFusarium species are devastating wheat pathogens. Plant growth-promoting rhizobacteria (PGPR) offer an effective biocontrol alternative. This study investigated the capacity of a Bacillus and Paenibacillus consortium to induce systemic resistance in winter wheat. MethodsWinter wheat plants were treated with a ten-strain PGPR consortium, with or without Fusarium oxysporum and F. graminearum. Expression of five PR-gene families was quantified in roots and shoots at 7 and 14 days post-treatment via RT-qPCR. Morphometric parameters were tracked for 28 days. ResultsThe consortium elicited a potent defense response. In roots at 7 days, PR-1 and PR-6 expression surged 16-fold and 38-fold, respectively. Defense activation in shoots was delayed, peaking at 14 days. After 28 days, PGPR-treated plants exhibited an 8% increase in shoot length. DiscussionThe results confirm that the bacterial strains effectively prime the wheat immune system, modulating early defense pathways and enhancing plant growth.

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Mekhantseva, K. V., Vasilchenko, N., Prazdnova, E., Usatov, A., Mazanko, M., Chistyakov, V.. 2025-10-16. Systemic induced plant resistance genes expression in winter wheat under treatment with antagonistic bacteria of Fusarium sp.. https://doi.org/10.1101/2025.10.16.682632

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