Mazanko, M. | bioRxiv Science

bioRxiv2025

Systemic induced plant resistance genes expression in winter wheat under treatment with antagonistic bacteria of Fusarium sp.

IntroductionFusarium species are devastating wheat pathogens. Plant growth-promoting rhizobacteria (PGPR) offer an effective biocontrol alternative. This study investigated the capacity of a Bacillus and Paenibacillus consortium to induce systemic resistance in winter wheat. MethodsWinter wheat plants were treated with a ten-strain PGPR consortium, with or without Fusarium oxysporum and F. graminearum. Expression of five PR-gene families was quantified in roots and shoots at 7 and 14 days post-treatment via RT-qPCR. Morphometric parameters were tracked for 28 days. ResultsThe consortium elicited a potent defense response. In roots at 7 days, PR-1 and PR-6 expression surged 16-fold and 38-fold, respectively. Defense activation in shoots was delayed, peaking at 14 days. After 28 days, PGPR-treated plants exhibited an 8% increase in shoot length. DiscussionThe results confirm that the bacterial strains effectively prime the wheat immune system, modulating early defense pathways and enhancing plant growth.

molecular biology↗

bioRxiv2024

Gellan gum-based nutrient medium increases the diversity of cultivated groups of soil microorganisms

Soil microbial communities contain a huge proportion of microorganisms that cannot be cultured on standard microbiological media and are available for study only by molecular methods such as metagenomics. Among them may be found microorganisms-producers of biologically active substances useful for medicine, biotechnology and agricultural activities of humans. Development of approaches for cultivation of such groups may open the way to the creation of fundamentally new antibiotic, antitumor drugs, phyto- and immunostimulants. We have proposed a solid oligotrophic medium for cultivation of soil microorganisms containing gellan gum, which allows to obtain cultures of more rare microorganisms compared to known media. Representatives of phylum Chloroflexota, Deinococcota, Planctomycetota, Actinomycetota, which were absent on agar media, were found in the metagenome of the community grown on this medium

microbiology↗