Comparison candidate Tick-borne encephalitis virus vaccines based on mRNA, adenovirus serotype 25 and chimera of YFV vaccine strain

Kuznetsova, N. A.; Gushchin, V. A.; Marchuk, E. V.; Siniavin, A.; Kleymenov, D. A.; Nikiforova, M.; Zubkova, O. V.; Vavilova, I. V.; Ozharovskaia, T. A.; Mazunina, E. P.; Bykonia, E. N.; Bogdanov, E. Y.; Zolotar, A. N.; Shidlovskaya, E. V.; Usachev, E. V.; Usacheva, O. V.; Shatalov, A. G.; Zlobin, V. I.; Logunov, D. Y.; Gintsburg, A. L.

doi:10.1101/2025.01.16.633390

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bioRxiv · 10.1101/2025.01.16.633390

Comparison candidate Tick-borne encephalitis virus vaccines based on mRNA, adenovirus serotype 25 and chimera of YFV vaccine strain

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Abstract

BackgroundDespite the availability of several licensed inactivated vaccines, the development of new vaccines against the Tick-borne encephalitis virus (TBEV) remains an important task, especially in countries endemic to this pathogen. The risk of infection with TBEV increases every year because of increase in the number of ticks, the emergence of tick carriers into new territories, and active human activity in areas of TBEV natural foci. Annual reports of vaccination failures have prompted us to search for approaches to creating a new vaccine. ObjectivesIn our study, we used the same PrM and E antigens to produce three vaccine candidates against tick-borne encephalitis (TBE): 1) a live attenuated YFV 17DD-UN vaccine strain, 2) recombinant adenovirus (rAd) vectors, and 3) mRNA encapsulated in lipid nanoparticles (LNP). MethodsWe generated and assessed the immunogenicity and protective efficacy of three candidate TBEV vaccines based on mRNA, simian adenovirus type 25, and a chimera of the YFV 17DD-UN attenuated strain. ResultsAnalysis of the virus-neutralizing titers in the blood sera of immunized mice revealed a statistically significant difference among the three candidate vaccines. The immunogenicity and protective efficacy of the candidate mRNA-LNP vaccine were found to be higher than those of the other two vaccines. ConclusionsBased on the results of our study, it can be concluded that the mRNA-based platform is more promising for the creation of a vaccine against TBEV.

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Kuznetsova, N. A., Gushchin, V. A., Marchuk, E. V., Siniavin, A., Kleymenov, D. A., Nikiforova, M., Zubkova, O. V., Vavilova, I. V., Ozharovskaia, T. A., Mazunina, E. P., Bykonia, E. N., Bogdanov, E. Y., Zolotar, A. N., Shidlovskaya, E. V., Usachev, E. V., Usacheva, O. V., Shatalov, A. G., Zlobin, V. I., Logunov, D. Y., Gintsburg, A. L.. 2025-01-21. Comparison candidate Tick-borne encephalitis virus vaccines based on mRNA, adenovirus serotype 25 and chimera of YFV vaccine strain. https://doi.org/10.1101/2025.01.16.633390

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