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bioRxiv · 10.1101/2024.10.31.621289

A lipid transport Mla Pqi Chimeric system is essential for Brucella abortus survival in macrophages

Lannoy, A.·Ronneau, A.·Fernandez Garcia, M.·Dieu, M.·Renard, P.·Garcia, A.·Conde, R.·De Bolle, X.
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Abstract

The envelope of diderm bacteria comprises of an inner membrane (IM) and an outer membrane (OM). Several pathways have been recently identified that facilitate the transport of phospholipids between the two membranes in Escherichia coli, including maintaining OM lipid asymmetry (Mla) and paraquat inducible (Pqi) systems. In this study, we report the identification and the characterization of a complex named Mpc in the intracellular pathogen Brucella abortus. Mpc is conserved in numerous species of Hyphomicrobiales and exhibits homology to both the Mla and Pqi systems. Mpc is essential for bacterial growth under conditions of envelope stress and for survival within macrophages during the early stages of infection. Analyses of protein-protein interactions and structural predictions indicate that the Mpc complex bridges IM to OM. The absence of this system results in an altered lipid composition of the OM vesicles, supporting the fact that Mpc plays a role in the transport of lipids between membranes. The discovery of a novel lipid trafficking system enhances the diversity and complexity of known lipid trafficking systems within diderm bacteria.

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Lannoy, A., Ronneau, A., Fernandez Garcia, M., Dieu, M., Renard, P., Garcia, A., Conde, R., De Bolle, X.. 2024-11-01. A lipid transport Mla Pqi Chimeric system is essential for Brucella abortus survival in macrophages. https://doi.org/10.1101/2024.10.31.621289

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