bioRxiv Science⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2024.06.28.601120

Long-term effects of ocean acidification and its interaction with warming on calcifying organisms and their associated microbiome: bryozoans as emerging sentinels of global change?

Figuerola, B.·Capdevila, P.·Cerda-Domenech, M.·Garrabou, J.·Mirasole, A.·Bassols, P.·del Campo, J.·Teixido, N.
View original source ↗Export metadata ↓

Abstract

Global ocean warming and acidification are two of the major threats to many marine calcifying habitat-forming species, potentially affecting entire ecosystems. Consequently, the need for a better understanding and predicting the response of marine calcifiers has never been more pressing. Paradoxically, the individual and combined long-term effects of these stressors on bryozoans have remained largely unexplored, despite their great abundance and diversity globally. Here, we first evaluate the changes in skeletal structure and mineralogy, and the associated microbiome composition on the populations of Pentapora ottomuelleriana (encrusting) and Myriapora truncata (erect) bryozoan species living inside and outside a volcanic CO2 vent in Ischia Island. We then examine the effects of a long-term exposure to elevated pCO2 and its combined effects of ocean warming on the proportion of cover of populations of the encrusting species through time after summer. Both bryozoan species show indicators of acclimatization by adjusting skeletal properties and having stable microbial communities under acidification conditions. However, we document novel patterns about microbiome shifts in response to future ocean acidification in bryozoans for the first time. Microbial genera known to have essential functions to the host such as biosynthesis of defense compounds or thermal protection were depleted at the acidified site, which suggest early warnings of potential deterioration of bryozoan health under near future ocean conditions. The proportion of cover of the encrusting species also decreased from 2016 to 2020 in both studied sites, with faster declines at the acidified ones. Our model suggests that the increasing seawater temperature drove a decline in the bryozoan cover although the combined effects with acidification accelerated its mortality rates in the CO2 vent. More multidisciplinary research combining both environmental stressors on a wider range of calcifying species is needed to better understand the adaptive capacity of the holobiont to a changing environment. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=187 SRC="FIGDIR/small/601120v1_ufig1.gif" ALT="Figure 1"> View larger version (98K): org.highwire.dtl.DTLVardef@1da75a5org.highwire.dtl.DTLVardef@161d3c6org.highwire.dtl.DTLVardef@196857eorg.highwire.dtl.DTLVardef@1161a2a_HPS_FORMAT_FIGEXP M_FIG C_FIG

Source connections

Explore related subjects

ecologyPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Figuerola, B., Capdevila, P., Cerda-Domenech, M., Garrabou, J., Mirasole, A., Bassols, P., del Campo, J., Teixido, N.. 2024-07-01. Long-term effects of ocean acidification and its interaction with warming on calcifying organisms and their associated microbiome: bryozoans as emerging sentinels of global change?. https://doi.org/10.1101/2024.06.28.601120

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Ecological opportunity and evolutionary relatedness predict parasite sharing differently across mammalian taxonomic scales

As global change brings species into new contact, understanding the ecological and evolutionary drivers of parasite sharing is essential for anticipating and mitigating disease emergence. Yet, the relative importance of geographic proximity and evolutionary relatedness among host species in predicting parasite sharing remains unresolved. We tested whether their relative importance changes across host taxonomic scale using 30,310 recorded associations between 680 mammalian and 11,107 parasite species. Geography accounted for 86.6% importance within genera, declining to 44.7% across all mammals, shifting toward evolutionary relatedness predominating. Parasite sharing increased nonlinearly with geographic proximity and host relatedness, and the overall trend varied among parasite types. Notably, accounting for host taxonomic scale improved parasite-sharing prediction accuracy. To explore the consequences of biotic homogenization, we applied a counterfactual scenario of reducing geographic distance by 99%, which increased mean predicted parasite sharing to 13.71% within genera and 1.50% at the class level. Together, these findings suggest that ecological opportunity is especially consequential among close relatives whereas evolutionary similarity is increasingly important among geographically distant species. Accounting for evolutionary scale can therefore improve sharing predictions and help identify which new host encounters warrant surveillance for cross-species transmission and disease emergence.

ecology↗
bioRxiv2026

Host availability, climate and pupal-stage ecology shape the predicted environmental suitability of the New World screwworm across Central and South America

Background: The New World screwworm, Cochliomyia hominivorax, causes primary myiasis in warm-blooded vertebrates. Its recent resurgence in Central America has renewed the need for spatial tools that identify environmentally suitable areas and surveillance gaps. Most previous distribution models have emphasized climate and livestock, whereas ecological constraints acting during the free-living pupal stage have rarely been incorporated. Methods: We compiled 2,461 georeferenced occurrence reports from the literature across Central and South America and linked them to climate, livestock density, forest cover and superficial soil predictors. Four sequential (nested) logistic models quantified the incremental contribution of predictor blocks, compared using likelihood-ratio tests, AIC and discrimination metrics. Logistic regression and Random Forest were then compared using five-fold spatial block cross-validation, which we treat as the primary measure of generalisation, and an independent municipality-level 70:30 train-test split. Variable importance was assessed using Random Forest Mean Decrease Accuracy together with partial-dependence profiles. Because presence and pseudo-absence classes were balanced before fitting, model outputs are interpreted as relative environmental suitability rather than absolute probability of occurrence. Results: The literature database represented 2,589 infected hosts, dominated by cattle, pigs, dogs and humans. Across the sequential models, AUC increased from 0.780 for climate alone to 0.898 with livestock density, 0.908 with forest cover and 0.918 with superficial soil properties; the increment attributable to the soil block ({Delta}AUC = 0.010) was smaller than the between-fold variability observed under spatial cross-validation (SD 0.049) and is therefore reported as suggestive rather than established. Sheep and horse density and cold-quarter soil temperature showed the highest Mean Decrease Accuracy values, while silt texture ranked above cattle density and forest cover. Under spatial block cross-validation Random Forest was markedly more stable than the GLM (mean AUC 0.926 {+/-} 0.049 versus 0.814 {+/-} 0.157) and was retained as the final model. On the independent municipality-level test set it achieved AUC 0.981, TSS 0.824, sensitivity 0.850 and specificity 0.972, values we regard as optimistic relative to the spatially blocked estimates. Predicted suitability was highest across Central America and parts of northern and tropical South America. Conclusions: C. hominivorax occurrence was associated with interacting climatic, host and landscape conditions. Superficial soil variables added a small, biologically interpretable signal consistent with constraints acting during pupation, but one that lies within the uncertainty of our spatial validation and requires confirmation with independent data. The maps estimate relative environmental suitability rather than infection incidence, and are intended to identify areas where surveillance could be strengthened rather than to guide control operations directly.

ecology↗
bioRxiv2026

Phenotypic variation in divergent mitochondrial haplotypes in migratory three-spined stickleback

Mitochondria are essential components in life-sustaining organismal processes. The mitochondrial genome can be influenced by selection, and divergent mitochondrial haplotypes have substantial potential to affect interspecies variation in life-history and physiology, yet little is known about whole organism phenotypic changes that might accompany mitochondrial genomic divergence. Three-spined stickleback on the Scottish island of North Uist provide a model to study this as their populations exhibit a well-mixed nuclear genome, yet two highly diverged mitochondrial haplotypes ( trans-Atlantic and European). Three-spined stickleback were sampled from six lochs to investigate associations between mitochondrial haplotype and phenotypic traits including organ size, body size, and ovule volume/clutch size, as well as citrate synthase enzyme activity. Results showed that mitochondrial haplotype has an influence on male gonadosomatic index (GSI) and potentially citrate synthase (CS) enzyme activity. Differences in male GSI may be explained by the timing of male spermatogenesis or potentially a need for larger testes in European haplotype fish. While no other phenotypic trait showed differences between mitochondrial haplotypes, there were many differences between sexes and populations e.g. in body size and organ/muscle size.

ecology↗