Biology subjects

Teixido, N.

Publications and source records attributed to Teixido, N..

3 recordsLinked to original sources

bioRxiv2026

Characterization of ovine follicular fluid and granulosa cell-derived extracellular vesicles and their miRNA cargo following in vitro exposure to bisphenols A and S.

Bisphenol A (BPA) and Bisphenol S (BPS) exposure disrupt ovarian function and granulosa cell (GC) steroidogenesis. Extracellular vesicles (EVs) and their miRNA cargo, as mediators of cellular response to environmental stimuli, might be involved in fertility and folliculogenesis. This study explored modulation of microRNA expression after 48h BPA or BPS exposure (10 {micro}M) in ovine primary GC and EVs from corresponding conditioned medium (CM EVs). Small RNA sequencing of control (0h) and 48h treated GC, CM EVs as well as follicular fluid EVs allowed identification of 533 ovine miRNAs, including 129 new sequences. BPA did not alter miRNA expression in GC, while BPS decreased cellular oar-24b miR. In contrast, BPA modified expression of 4 miRNAs in CM-EVs, including 3 new sequences, and two miRNAs were modified by BPS. Both compounds reduced expression of sequence homologous to miR-1306. Further studies are required to decipher their roles in bisphenol toxicity in GC.

molecular biology↗

bioRxiv2024

Aboral cell types of Clytia and coral larvae have shared features and link taurine to the regulation of settlement

Planktonic larvae of many marine invertebrates settle on a suitable substrate and metamorphose into bottom-dwelling adults. Larval settlement is of considerable interest both for ecologists and for evolutionary biologists, who have proposed that anterior sensory systems for substrate selection provided the basis for animal brains. Nevertheless the cellular and molecular regulation of larval settlement, including in Cnidaria (corals, jellyfish, sea anemones, hydroids) is not well understood. We generated and compared anterior (aboral) transcriptomes and single-cell RNA-seq datasets from the planula larvae of three cnidarian species: the hydrozoan jellyfish Clytia hemisphaerica, and the scleractinian corals Astroides calycularis and Pocillopora acuta. Integrating these datasets and characterizing aboral cell types, we defined a common cellular architecture of the planula aboral end, and identified clade-specific specializations in cell types, including unique aboral neural cells in the Clytia planula and neurosecretory cell types with distinct molecular signatures in both Clytia and coral planulae. Among common planula aboral features were genes implicated in taurine uptake and catabolism expressed in distinct specialized cell types. In functional assays, exogenous taurine inhibited settlement of both Clytia and Astroides planulae. These findings define a detailed molecular and cellular framework of the planula aboral pole, and implicate localized taurine destruction in defining settlement competence.

evolutionary biology↗

bioRxiv2024

Long-term effects of ocean acidification and its interaction with warming on calcifying organisms and their associated microbiome: bryozoans as emerging sentinels of global change?

Global ocean warming and acidification are two of the major threats to many marine calcifying habitat-forming species, potentially affecting entire ecosystems. Consequently, the need for a better understanding and predicting the response of marine calcifiers has never been more pressing. Paradoxically, the individual and combined long-term effects of these stressors on bryozoans have remained largely unexplored, despite their great abundance and diversity globally. Here, we first evaluate the changes in skeletal structure and mineralogy, and the associated microbiome composition on the populations of Pentapora ottomuelleriana (encrusting) and Myriapora truncata (erect) bryozoan species living inside and outside a volcanic CO2 vent in Ischia Island. We then examine the effects of a long-term exposure to elevated pCO2 and its combined effects of ocean warming on the proportion of cover of populations of the encrusting species through time after summer. Both bryozoan species show indicators of acclimatization by adjusting skeletal properties and having stable microbial communities under acidification conditions. However, we document novel patterns about microbiome shifts in response to future ocean acidification in bryozoans for the first time. Microbial genera known to have essential functions to the host such as biosynthesis of defense compounds or thermal protection were depleted at the acidified site, which suggest early warnings of potential deterioration of bryozoan health under near future ocean conditions. The proportion of cover of the encrusting species also decreased from 2016 to 2020 in both studied sites, with faster declines at the acidified ones. Our model suggests that the increasing seawater temperature drove a decline in the bryozoan cover although the combined effects with acidification accelerated its mortality rates in the CO2 vent. More multidisciplinary research combining both environmental stressors on a wider range of calcifying species is needed to better understand the adaptive capacity of the holobiont to a changing environment. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=187 SRC="FIGDIR/small/601120v1_ufig1.gif" ALT="Figure 1"> View larger version (98K): org.highwire.dtl.DTLVardef@1da75a5org.highwire.dtl.DTLVardef@161d3c6org.highwire.dtl.DTLVardef@196857eorg.highwire.dtl.DTLVardef@1161a2a_HPS_FORMAT_FIGEXP M_FIG C_FIG

ecology↗