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bioRxiv · 10.1101/2024.06.04.596536

Accelerating CAR T cell manufacturing with an automated next-day process

Ahmadi, M.·Putnam, N.·Hayoun, D.·Dotson, M.·Padilla, J.·Fatima, N.·Nonterah, G.·de Mollerat du Jeu, X.·Ji, Y.
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Abstract

The traditional method of CAR T cell production involves lengthy ex vivo culture times which can result in the reduction of crucial naive T cell subsets. Moreover, traditional CAR T cell therapy manufacturing processes can prolong time-to-patient and contribute to disease progression. In this study, we describe an innovative and automated 24-hour CAR T manufacturing process that yields a higher percentage of naive/stem-cell like T cells which have increased cytotoxic activity and cytokine release. The data supports the feasibility of implementing this streamlined manufacturing process in clinics. This approach also has the potential to enhance CAR T therapy efficacy and improve patient access to therapy.

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Ahmadi, M., Putnam, N., Hayoun, D., Dotson, M., Padilla, J., Fatima, N., Nonterah, G., de Mollerat du Jeu, X., Ji, Y.. 2024-06-06. Accelerating CAR T cell manufacturing with an automated next-day process. https://doi.org/10.1101/2024.06.04.596536

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