Biology subjects

Putnam, N.

Publications and source records attributed to Putnam, N..

2 recordsLinked to original sources

bioRxiv2025

In vivo evolution of Candida auris multi-drug resistance in a patient receiving antifungal treatment

Candida auris is associated with life-threatening invasive disease due to high level of drug resistance. We present a clinical case of C. auris multi-drug resistance development in a single patient acquired during antifungal treatment. Five isolates were prospectively recovered from a transplant patient receiving antifungal therapy over a one-year period. While isolates were initially only resistant to fluconazole, the terminal isolate became resistant to caspofungin and amphotericin B with significant increase in micafungin MIC. Sequencing of ERG11 and FKS1 genes identified mutations associated with fluconazole and echinocandin resistance in the multi-drug-resistant isolate, underscoring the threat of therapy-induced development of resistance.

microbiology↗

bioRxiv2024

Accelerating CAR T cell manufacturing with an automated next-day process

The traditional method of CAR T cell production involves lengthy ex vivo culture times which can result in the reduction of crucial naive T cell subsets. Moreover, traditional CAR T cell therapy manufacturing processes can prolong time-to-patient and contribute to disease progression. In this study, we describe an innovative and automated 24-hour CAR T manufacturing process that yields a higher percentage of naive/stem-cell like T cells which have increased cytotoxic activity and cytokine release. The data supports the feasibility of implementing this streamlined manufacturing process in clinics. This approach also has the potential to enhance CAR T therapy efficacy and improve patient access to therapy.

bioengineering↗