A duplexed CRISPRi-based approach to predict synergistic antibiotic targets in Escherichia coli.

Xiong, X.; Furseth, H.; McCoy, B.; Slavic, L.; Yuan, X.; Chen, S.; Baughn, A. D.; Minato, Y.; Harcombe, W. R.

doi:10.1101/2024.05.15.594409

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bioRxiv · 10.1101/2024.05.15.594409

A duplexed CRISPRi-based approach to predict synergistic antibiotic targets in Escherichia coli.

Xiong, X.·Furseth, H.·McCoy, B.·Slavic, L.·Yuan, X.·Chen, S.·Baughn, A. D.·Minato, Y.·Harcombe, W. R.

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Abstract

Co-repressing drug targets with CRISPR interference (CRISPRi) has the potential to predict both synergistic and additive drug combinations. In a screen experiment with Escherichia coli, pairwise gene co-repression assays correctly predicted the interaction type observed for 36 antibiotic pairs in checkerboard assays. Furthermore, we used this approach to identify promising synergistic interactions with a gene currently not targeted by clinical antibiotics. CRISPRi can systematically test the impact of gene inhibitions and identify novel drug targets.

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Xiong, X., Furseth, H., McCoy, B., Slavic, L., Yuan, X., Chen, S., Baughn, A. D., Minato, Y., Harcombe, W. R.. 2024-05-16. A duplexed CRISPRi-based approach to predict synergistic antibiotic targets in Escherichia coli.. https://doi.org/10.1101/2024.05.15.594409

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