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bioRxiv · 10.1101/2024.03.21.585958

34-parameter full spectrum immunophenotyping panel of human regulatory and effector lymphocytes

Georgieva, Z.·Coppard, V.·Yang, J. H.·Grenfell, R.·Jones, J. L.
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Abstract

This 34-marker sentinel, intracellular, full-spectrum flow cytometry panel profiles regulatory and effector T, B and NK lymphocytes in human cryopreserved peripheral blood mononuclear cells. The panel focuses on cell trafficking, activation, exhaustion and proliferation, and permits easy customisation in two positions to accommodate other targets of the users interest. By combining breadth and depth of phenotyping, this panel is designed to maximise the information obtained from limited cell material and therefore will be particularly useful in mechanistic studies of immunomodulatory drugs for autoimmune disease, cancer and transplantation, where multiple immune populations may be affected.

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Georgieva, Z., Coppard, V., Yang, J. H., Grenfell, R., Jones, J. L.. 2024-03-27. 34-parameter full spectrum immunophenotyping panel of human regulatory and effector lymphocytes. https://doi.org/10.1101/2024.03.21.585958

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