SHIELD: Skull-shaped hemispheric implants enabling large-scale-electrophysiology datasets in the mouse brain

Bennett, C.; Ouellette, B.; Ramirez, T. K.; Cahoon, A.; Cabasco, H.; Belski, H.; Gillis, R.; Grasso, C.; Howard, R.; Johnson, T.; Loeffler, H.; Smith, H.; Sullivan, D.; Williford, A.; Caldejon, S.; Durand, S.; Gale, S. D.; Guthrie, A.; Ha, V.; Han, W.; Hardcastle, B.; McBride, E.; Mochizuki, C.; Sridhar, A.; Suarez, L.; Swapp, J.; Wilkes, J.; Farrell, C.; Groblewski, P.; Olsen, S.

doi:10.1101/2023.11.12.566771

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bioRxiv · 10.1101/2023.11.12.566771

SHIELD: Skull-shaped hemispheric implants enabling large-scale-electrophysiology datasets in the mouse brain

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Abstract

To understand the neural basis of behavior, it is essential to measure spiking dynamics across many interacting brain regions. While new technologies, such as Neuropixels probes, facilitate multi-regional recordings, significant surgical and procedural hurdles remain for these experiments to achieve their full potential. Here, we describe a novel 3D-printed cranial-replacement implant (SHIELD) enabling electrophysiological recordings from distributed areas of the mouse brain. This skull-shaped implant is designed with customizable insertion holes, allowing dozens of cortical and subcortical structures to be recorded in a single mouse using repeated multi-probe insertions over many days. We demonstrate the procedures high success rate, biocompatibility, lack of adverse effects on behavior, and compatibility with imaging and optogenetics. To showcase the scientific utility of the SHIELD implant, we use multi-probe recordings to reveal novel insights into how alpha rhythms organize spiking activity across visual and sensorimotor networks. Overall, this method enables powerful large-scale electrophysiological measurements for the study of distributed brain computation.

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Bennett, C., Ouellette, B., Ramirez, T. K., Cahoon, A., Cabasco, H., Belski, H., Gillis, R., Grasso, C., Howard, R., Johnson, T., Loeffler, H., Smith, H., Sullivan, D., Williford, A., Caldejon, S., Durand, S., Gale, S. D., Guthrie, A., Ha, V., Han, W., Hardcastle, B., McBride, E., Mochizuki, C., Sridhar, A., Suarez, L., Swapp, J., Wilkes, J., Farrell, C., Groblewski, P., Olsen, S.. 2023-11-14. SHIELD: Skull-shaped hemispheric implants enabling large-scale-electrophysiology datasets in the mouse brain. https://doi.org/10.1101/2023.11.12.566771

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