Sperm Cell Painting: A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa

Barratt, C. L. R.; Johnston, Z. C.; Gruber, F. S.; Swedlow, J. R.; Myles, R.; Kane, S. V.; Richardson, A.; Wilson, C.; Martins da Silva, S.; Gilbert, I. H.; Read, K. D.; Day, D. P.

doi:10.1101/2023.09.15.557919

bioRxiv · 10.1101/2023.09.15.557919

Sperm Cell Painting: A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa

Barratt, C. L. R.·Johnston, Z. C.·Gruber, F. S.·Swedlow, J. R.·Myles, R.·Kane, S. V.·Richardson, A.·Wilson, C.·Martins da Silva, S.·Gilbert, I. H.·Read, K. D.·Day, D. P.

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Abstract

We have adapted the cell painting assay developed by Carpenter and colleagues on cultured U2OS cells to human spermatozoa. In Sperm Cell Painting (SCP) we assemble an image-based quantitative fingerprint of the functional state of sperm. We use this assay to gain insight into the mechanism of action of compounds that modify sperm function and as a platform for contraceptive discovery.

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Barratt, C. L. R., Johnston, Z. C., Gruber, F. S., Swedlow, J. R., Myles, R., Kane, S. V., Richardson, A., Wilson, C., Martins da Silva, S., Gilbert, I. H., Read, K. D., Day, D. P.. 2023-09-17. Sperm Cell Painting: A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa. https://doi.org/10.1101/2023.09.15.557919

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