Biology subjects

Myles, R.

Publications and source records attributed to Myles, R..

2 recordsLinked to original sources

bioRxiv2023

Sperm Cell Painting: A Mechanism Driven Approach for Drug Discovery in Human Spermatozoa

We have adapted the cell painting assay developed by Carpenter and colleagues on cultured U2OS cells to human spermatozoa. In Sperm Cell Painting (SCP) we assemble an image-based quantitative fingerprint of the functional state of sperm. We use this assay to gain insight into the mechanism of action of compounds that modify sperm function and as a platform for contraceptive discovery.

cell biology↗

bioRxiv2023

Sperm Toolbox - A selection of small molecules to study human spermatozoa

Male contraceptive options and infertility treatments are limited. To help overcome these limitations we established a small molecule library as a toolbox for assay development and screening campaigns using human spermatozoa. We have profiled all compounds in our automated high-throughput screening platform and provide the dataset, which allows direct side-by-side comparisons of compound effects and assays using live human spermatozoa.

pharmacology and toxicology↗