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bioRxiv · 10.1101/2023.09.07.554570

Stereotypic persistent B cell receptor clonotypes in Alzheimer's Disease

Yang, H.·Kim, N.·Lee, Y.·Yoo, D. K.·Choi, J.·Kim, K.·Bae, J.·Han, J.·Kwon, S.·Chung, J.
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Abstract

We constructed B cell receptor (BCR) repertoires in silico using peripheral blood (PB) samples collected from 44 Alzheimers Disease (AD) patients at baseline and 37 patients at follow-up. For the control group (CG), we used BCR repertoire data from the chronologically collected PB samples of 55 healthy volunteers vaccinated with SARS-CoV-2 mRNA. The AD patients shared 3,983 stereotypic non-naive BCR clonotypes not found in CG, and their degree of overlap between patient pairs were significantly higher than that of CG pairs, even with the SARS-CoV-2 spike protein triggering a concerted BCR response. Twenty stereotypic non-naive AD patient-specific BCR clonotypes co-existed in more than four patients and persisted throughout two sampling points. One of these BCR clonotypes encoded an antibody reactive to the A{beta}42 peptide. Our findings strongly suggest that AD patients are exposed to common (auto)antigens associated with disease pathology, and their BCR repertoires show unique signatures with diagnostic potential.

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Yang, H., Kim, N., Lee, Y., Yoo, D. K., Choi, J., Kim, K., Bae, J., Han, J., Kwon, S., Chung, J.. 2023-09-08. Stereotypic persistent B cell receptor clonotypes in Alzheimer's Disease. https://doi.org/10.1101/2023.09.07.554570

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