Time-deterministic cryo-optical microscopy

Yamanaka, M.; Tsuji, K.; Kumamoto, Y.; Tamura, S.; Miyamura, W.; Kubo, T.; Mizushima, K.; Kono, K.; Hirano, H.; Sugiura, K.; Fukushima, S.-i.; Kunimoto, T.; Nishida, K.; Mochizuki, K.; Harada, Y.; Smith, N. I.; Heintzmann, R.; Nagai, T.; Tanaka, H.; Fujita, K.

doi:10.1101/2023.08.01.551103

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bioRxiv · 10.1101/2023.08.01.551103

Time-deterministic cryo-optical microscopy

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Abstract

We developed a technique to rapidly freeze biological systems during observation under an optical microscope, which allows for detailed optical observation at a precise time in the biological dynamics at high spatial resolution and quantifiability under low-temperature conditions. Our study further found that ion distributions and molecular conformations can be fixed in space and time, demonstrated by visualization of calcium waves rapid-frozen at an instant using fluorescent ion indicators. These results show that time-deterministic cryo-optical microscopy can suspend cellular dynamics while maintaining molecular and ionic states, allowing us to view cellular dynamics in a way that has not been possible before. One-Sentence SummaryRapid freezing of dynamic biological systems during optical observation presents time information to the fixed samples

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Yamanaka, M., Tsuji, K., Kumamoto, Y., Tamura, S., Miyamura, W., Kubo, T., Mizushima, K., Kono, K., Hirano, H., Sugiura, K., Fukushima, S.-i., Kunimoto, T., Nishida, K., Mochizuki, K., Harada, Y., Smith, N. I., Heintzmann, R., Nagai, T., Tanaka, H., Fujita, K.. 2023-08-03. Time-deterministic cryo-optical microscopy. https://doi.org/10.1101/2023.08.01.551103

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