Stability of mpox (monkeypox) virus in bodily fluids and wastewater

Yinda, C. K.; Morris, D. H.; Fischer, R.; Gallogly, S.; Weishampel, Z.; Port, J. R.; Bushmaker, T.; Schulz, J. E.; Bibby, K.; van Doremalen, N.; Lloyd-Smith, J. O.; Munster, V.

doi:10.1101/2023.05.09.540015

bioRxiv · 10.1101/2023.05.09.540015

Stability of mpox (monkeypox) virus in bodily fluids and wastewater

Yinda, C. K.·Morris, D. H.·Fischer, R.·Gallogly, S.·Weishampel, Z.·Port, J. R.·Bushmaker, T.·Schulz, J. E.·Bibby, K.·van Doremalen, N.·Lloyd-Smith, J. O.·Munster, V.

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Abstract

ImportanceSince May 2022, human monkeypox (mpox) infections have spread rapidly outside endemic countries. On July 23, 2023, WHO declared a Public Health Emergency of international Concern because of the unprecedented global spread of mpox. Whereas there is an incomplete understanding of transmission routes, the spread of monkeypox virus (MPXV) through sexual contact networks of men who have sex with men (MSM) highlights the potential of transmission during sexual activity, either via bodily fluids or via direct contact. This research assesses the potential for MPXV to remain infectious in the environment on surfaces and in fluids under different environmental conditions. ObjectiveTo investigate the stability of MPXV on various surfaces, at different environmental conditions and in different bodily fluids (human blood, semen, serum, saliva, urine, and feces), and to assess decontamination of MPXV-contaminated wastewater via chlorination. Design, Setting, and ExposuresAll experiments involving viable MPXV were performed at the Rocky Mountains Laboratory under high containment conditions using MPXV strain hMPXV/USA/MA001. Infectious MPXV was quantified via plaque assay. Environmental decay rates were estimated using a Bayesian statistical model, with a Poisson likelihood for plaque counts. ResultsMPXV showed surface dependent stability, with faster decay at higher temperatures. Decay rates varied considerably depending on the medium in which virus was suspended, both overall MPXV displayed considerable environmental stability in bodily fluids and in particular proteinaceous fluids, such as blood and semen, lead to greater persistence. Wastewater chlorination was an effective decontamination technique. Conclusions and RelevanceMPXV retains its infectivity in the environment when it is deposited in blood, semen, and serum; environmental decay is more rapid in less proteinaceous fluids, particularly once residual liquid has evaporated. The observed persistence of MPXV implies environmental contamination in healthcare setting, could serve as vehicles of transmission and dissemination in these environments. Chlorination was effective in decontamination of wastewater. Key findings and public health implications; these results suggest that MPXV can have prolonged stability in the environment and are therefore directly relevant for hospital hygiene practices to prevent nosocomial transmission.

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Yinda, C. K., Morris, D. H., Fischer, R., Gallogly, S., Weishampel, Z., Port, J. R., Bushmaker, T., Schulz, J. E., Bibby, K., van Doremalen, N., Lloyd-Smith, J. O., Munster, V.. 2023-05-09. Stability of mpox (monkeypox) virus in bodily fluids and wastewater. https://doi.org/10.1101/2023.05.09.540015

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