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bioRxiv · 10.1101/2023.01.30.526196

TGF-β downregulates antigen processing and presentation genes and MHC I surface expression through a Smad3-dependent mechanism.

Berglund, A. K.·Hinson, A. L.·Schnabel, L. V.
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Abstract

Regulation of MHC I surface expression by TGF-{beta} is important for controlling cell-mediated immune responses, but how TGF-{beta} downregulates MHC I is unknown. We investigated this mechanism using flow cytometry and RNA-sequencing to identify the major TGF-{beta} signaling pathway and target genes involved. All three isoforms of TGF-{beta} were found to have similar abilities to downregulate constitutive MHC I surface expression. Inhibiting the type I TGF-{beta} receptor, ALK5, as well the canonical TGF-{beta} signaling molecule Smad3, prevented TGF-{beta} from downregulating MHC I surface expression. RNA-sequencing from horse and human mesenchymal stem cells revealed that multiple genes associated with antigen processing and presentation were downregulated in TGF-{beta}-treated cells with B2M and ERAP1 being downregulated in both species. While downregulation of MHC I surface expression a slow process that continued past 24 hours after TGF-{beta} treatment, downregulation of gene expression of ERAP1 occurred within 12 hours after treatment. B2M expression was not consistently downregulated until after 24 hours and TAP2 expression was inconsistent over a 72-hour period, although it was significantly downregulated at 12 hours after TGF-{beta} treatment. This data supports that TGF-{beta} downregulates antigen processing and presentation genes resulting in decreased surface expression of MHC I through a Smad3-dependent mechanism.

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Berglund, A. K., Hinson, A. L., Schnabel, L. V.. 2023-02-01. TGF-β downregulates antigen processing and presentation genes and MHC I surface expression through a Smad3-dependent mechanism.. https://doi.org/10.1101/2023.01.30.526196

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