The Structural Layers of the Porcine Iris Exhibit Inherently Different Biomechanical Properties

Tan, R. K. Y.; Panda, S. K.; Braeu, F. A.; Muralidharan, A. R.; Nongpiur, M. E.; Chan, A. S. Y.; Aung, T.; Najjar, R. P.; Girard, M. J. A.

doi:10.1101/2022.12.11.519999

bioRxiv · 10.1101/2022.12.11.519999

The Structural Layers of the Porcine Iris Exhibit Inherently Different Biomechanical Properties

Tan, R. K. Y.·Panda, S. K.·Braeu, F. A.·Muralidharan, A. R.·Nongpiur, M. E.·Chan, A. S. Y.·Aung, T.·Najjar, R. P.·Girard, M. J. A.

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Abstract

PurposeTo isolate the structural components of the ex vivo porcine iris tissue and to determine their biomechanical properties. MethodsThe porcine stroma and dilator tissues were separated, and their dimensions were assessed using optical coherence tomography (OCT). The stroma underwent flow test (n = 32) to evaluate for permeability using Darcys Law ({Delta}P = 2000 Pa, A = 0.0391 mm2), and both tissues underwent stress relaxation experiments ({varepsilon} = 0.5 with initial ramp of {delta}{varepsilon} = 0.1) to evaluate for their viscoelastic behaviours (n = 28). Viscoelasticity was characterised by the parameters {beta} (half width of the Gaussian distribution), {tau}m(mean relaxation time constant), E0 (instantaneous modulus) and E{infty} (equilibrium modulus). ResultsFor the stroma, the hydraulic permeability was 9.49 {+/-} 3.05 x 10-6 mm2/Pa{middle dot}s, and the viscoelastic parameters were {beta} = 2.50 {+/-} 1.40, and {tau}m = 7.43 {+/-} 4.96 s, with the two moduli calculated to be E0= 14.14 {+/-} 6.44 kPa and E{infty} = 6.08 {+/-} 2.74 kPa. For the dilator tissue, the viscoelastic parameters were {beta} = 2.06 {+/-} 1.33 and {tau}m = 1.28 {+/-} 1.27 s, with the two moduli calculated to be E0 = 9.16 {+/-} 3.03 kPa and E{infty} = 5.54 {+/-} 1.98 kPa. ConclusionWe have established a new protocol to evaluate the biomechanical properties of the structural layers of the iris. Overall, the stroma was permeable and exhibited smaller moduli than those of the dilator muscle. An improved characterisation of iris biomechanics may form the basis to further our understanding of angle closure glaucoma.

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Tan, R. K. Y., Panda, S. K., Braeu, F. A., Muralidharan, A. R., Nongpiur, M. E., Chan, A. S. Y., Aung, T., Najjar, R. P., Girard, M. J. A.. 2022-12-14. The Structural Layers of the Porcine Iris Exhibit Inherently Different Biomechanical Properties. https://doi.org/10.1101/2022.12.11.519999

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