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bioRxiv · 10.1101/2022.10.20.512862

Genome sequencing of the Monkeypox virus 2022 outbreak with Amplicon-based Oxford Nanopore MinION sequencing

Brinkmann, A.·Pape, K.·Uddin, S.·Woelk, N.·Foerster, S.·Jessen, H.·Michel, J.·Kohl, C.·Schaade, L.·Nitsche, A.
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Abstract

We present an amplicon-based assay for MinION Nanopore sequencing of monkeypox virus genomes from clinical specimens, obtaining high-quality results for up to 99 % of the genomes, even for samples with high Ct values of up to 30 which are not suitable for shotgun sequencing.

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Brinkmann, A., Pape, K., Uddin, S., Woelk, N., Foerster, S., Jessen, H., Michel, J., Kohl, C., Schaade, L., Nitsche, A.. 2022-10-20. Genome sequencing of the Monkeypox virus 2022 outbreak with Amplicon-based Oxford Nanopore MinION sequencing. https://doi.org/10.1101/2022.10.20.512862

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