Genome sequencing of the Monkeypox virus 2022 outbreak with Amplicon-based Oxford Nanopore MinION sequencing
We present an amplicon-based assay for MinION Nanopore sequencing of monkeypox virus genomes from clinical specimens, obtaining high-quality results for up to 99 % of the genomes, even for samples with high Ct values of up to 30 which are not suitable for shotgun sequencing.
molecular biology↗