Inference of evolutionary transitions to self-fertilization using whole-genome sequences

Abstract

The evolution from outcrossing to selfing is a transition that occurred recurrently throughout the eukaryote tree of life, in plants, animals, fungi and algae. Despite some short-term advantages, selfing is supposed to be an evolutionary dead-end reproductive strategy on the long-term and its tippy distribution on phylogenies suggests that most selfing species are of recent origin. However, dating such transitions is challenging while it is central for this hypothesis. We develop two methods making use of full genome polymorphism data to 1) test if a transition from outcrossing to selfing occurred, and 2) infer its age. The sequentially Markov coalescent based (teSMC) and the Approximate Bayesian Computation (tsABC) methods use a common framework based on a transition matrix summarizing the distribution of times to the most recent common ancestor along the genome, allowing to estimate changes in the ratio of population recombination and mutation rates in time. We first demonstrate that our methods can disentangle between past change in selfing rate from past changes in demographic history. Second, we assess the accuracy of our methods and show that transitions to selfing as old as approximatively 2.5Ne generations can be identified from polymorphism data. Third, our estimates are robust to the presence of linked negative selection on coding sequences. Finally, as a proof of principle, we apply both methods to three populations from Arabidopsis thaliana, recovering a transition to selfing which occurred approximately 600,000 years ago. Our methods pave the way to study recent transitions to predominant self-fertilization in selfing organisms. Significance statementSelf-fertilization evolved recurrently from outcrossing in many groups of organisms. When, why, and at what pace such transitions occurred are central to understand the evolution of reproductive systems but dating them remains highly challenging. While experimental work can be conducted in ecological set-ups, it is difficult to reconstruct and empirically test the past ecological conditions which could have driven transitions from outcrossing to self-fertilizing reproduction. We suggest here to use full genome data of several individuals per population to estimate if and when a transition in reproductive strategy occurred. We develop two methods which can be applied to estimate the age of such transitions jointly with the species demographic history.