High-resolution line-scan Brillouin microscopy for live-imaging of mechanical properties during embryo development

Bevilacqua, C.; Gomez, J. M.; Fiuza, U.-M.; Chan, C. J.; Wang, L.; Hambura, S.; Eguren, M.; Ellenberg, J.; Diz-Munoz, A.; Leptin, M.; Prevedel, R.

doi:10.1101/2022.04.25.489364

bioRxiv · 10.1101/2022.04.25.489364

High-resolution line-scan Brillouin microscopy for live-imaging of mechanical properties during embryo development

Bevilacqua, C.·Gomez, J. M.·Fiuza, U.-M.·Chan, C. J.·Wang, L.·Hambura, S.·Eguren, M.·Ellenberg, J.·Diz-Munoz, A.·Leptin, M.·Prevedel, R.

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Abstract

Brillouin microscopy (BM) can be used to assess the mechanical properties of biological samples in a 3D, all-optical, and hence non-contact fashion, but its weak signals require long imaging times and illumination dosages harmful to living organisms. Here, we present a line-scanning Brillouin microscope optimized for fast and high-resolution live-imaging of dynamic biological processes with low photo-toxicity. In combination with fluorescence light-sheet imaging, we demonstrate the capabilities of our microscope to visualize the mechanical properties of cells and tissues over space and time in living model organisms such as fruit flies, ascidians, and mouse embryos.

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Bevilacqua, C., Gomez, J. M., Fiuza, U.-M., Chan, C. J., Wang, L., Hambura, S., Eguren, M., Ellenberg, J., Diz-Munoz, A., Leptin, M., Prevedel, R.. 2022-04-26. High-resolution line-scan Brillouin microscopy for live-imaging of mechanical properties during embryo development. https://doi.org/10.1101/2022.04.25.489364

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