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bioRxiv · 10.1101/2022.04.18.488640

BCG vaccination of Diversity Outbred mice induces cross-reactive antibodies to SARS-CoV-2 spike protein

Specht, A. G.·Kurtz, S. L.·Elkins, K. L.·Specht, H.·Beamer, G.
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Abstract

The Bacillus Calmette-Guerin (BCG) vaccine, the only vaccine against tuberculosis, induces cross-protection against pathogens unrelated to Mycobacterium, including viruses. Epidemiological studies have identified potential benefits of BCG vaccination against SARS-CoV-2 infection. While BCGs heterologous effects have been widely attributable to trained immunity, we hypothesized BCG vaccination could induce cross-reactive antibodies against the spike protein of SARS-CoV-2 Wuhan-Hu-1. The concentration of IgG reactive to SARS-CoV-2 spike protein from the sera of BCG-vaccinated, Diversity Outbred (DO) mice and C57BL/6J inbred mice was measured using ELISA. Sera from 10/15 BCG-vaccinated DO mice possessed more IgG reactive to recombinant spike protein than sera from BCG-vaccinated C57BL/6J mice and unvaccinated DO mice. Amino acid sequences common to BCG cell wall/membrane proteins and SARS-CoV-2 spike protein were identified as potential antigen candidates for future study. These results imply a humoral mechanism, influenced by genotype, by which BCG vaccination could confer immunity to SARS-CoV-2. Graphic Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=80 SRC="FIGDIR/small/488640v1_ufig1.gif" ALT="Figure 1"> View larger version (23K): org.highwire.dtl.DTLVardef@1ff9d16org.highwire.dtl.DTLVardef@a2302dorg.highwire.dtl.DTLVardef@8ee2borg.highwire.dtl.DTLVardef@4c7b55_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Specht, A. G., Kurtz, S. L., Elkins, K. L., Specht, H., Beamer, G.. 2022-04-19. BCG vaccination of Diversity Outbred mice induces cross-reactive antibodies to SARS-CoV-2 spike protein. https://doi.org/10.1101/2022.04.18.488640

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