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bioRxiv · 10.1101/2022.01.12.476007

A whole-cell recording database of neuromodulatory action in the adult neocortex

Yan, X.·Calcini, N.·Safavi, P.·Ak, A.·Kole, K.·Zeldenrust, F.·Celikel, T.
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Abstract

BackgroundThe recent release of two large intracellular electrophysiological databases now allows high-dimensional systematic analysis of mechanisms of information processing in the neocortex. Here, to complement these efforts, we introduce a freely and publicly available database that provides a comparative insight into the role of various neuromodulatory transmitters in controlling neural information processing. FindingsA database of in vitro whole-cell patch-clamp recordings from primary somatosensory and motor cortices (layers 2/3) of the adult mice (2-15 months old) from both sexes is introduced. A total of 464 current-clamp experiments from identified excitatory and inhibitory neurons are provided. Experiments include recordings with (i) Step-and-Hold protocol during which the current was transiently held at 10 steps, gradually increasing in amplitude, (ii) "Frozen Noise" injections that model the amplitude and time-varying nature of synaptic inputs to a neuron in biological networks. All experiments follow a within neuron across drug design which includes a vehicle control and a modulation of one of the following targets in the same neuron: dopamine and its receptors D1R, D2R, serotonin 5HT1f receptor, norepinephrine Alpha1, and acetylcholine M1 receptors. ConclusionsThis dataset is the first to provide a systematic and comparative insight into the role of the selected neuromodulators in controlling cellular excitability. The data will help to mechanistically address how bottom-up information processing can be modulated, providing a reference for studying neural coding characteristics and revealing the contribution of neuromodulation to information processing.

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Yan, X., Calcini, N., Safavi, P., Ak, A., Kole, K., Zeldenrust, F., Celikel, T.. 2022-01-13. A whole-cell recording database of neuromodulatory action in the adult neocortex. https://doi.org/10.1101/2022.01.12.476007

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