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bioRxiv · 10.1101/2021.12.15.472793

Dynamic epistasis analysis reveals how chromatin remodeling regulates transcriptional bursting

Brouwer, I.·Kerklingh, E.·van Leeuwen, F.·Lenstra, T. L.
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Abstract

Transcriptional bursting has been linked to the stochastic positioning of nucleosomes. However, how bursting is regulated by remodeling of promoter nucleosomes is unknown. Here, we use single-molecule live-cell imaging of GAL10 transcription in budding yeast to measure how transcriptional bursting changes upon single and double perturbations of chromatin remodeling factors, the transcription factor Gal4 and preinitiation complex (PIC) components. Using dynamic epistasis analysis, we reveal how remodeling of different nucleosomes regulates individual transcriptional bursting parameters. At the nucleosome covering the Gal4 binding sites, RSC acts synergistically with Gal4 binding to facilitate each burst. Conversely, nucleosome remodeling at the TATA box controls only the first burst upon galactose induction. In the absence of remodelers, nucleosomes at canonical TATA boxes are displaced by TBP binding to allow for transcription activation. Overall, our results reveal how promoter nucleosome remodeling, together with transcription factor and PIC binding regulates the kinetics of transcriptional bursting.

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Brouwer, I., Kerklingh, E., van Leeuwen, F., Lenstra, T. L.. 2021-12-15. Dynamic epistasis analysis reveals how chromatin remodeling regulates transcriptional bursting. https://doi.org/10.1101/2021.12.15.472793

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