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bioRxiv · 10.1101/2021.12.10.472140

High-speed, multi-Z confocal microscopy for voltage imaging in densely labeled neuronal populations

Weber, T. D.·Moya, M. V.·Mertz, J.·Economo, M. N.
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Abstract

Genetically encoded voltage indicators (GEVIs) hold great promise for monitoring neuronal population activity, but GEVI imaging in dense neuronal populations remains difficult due to a lack of contrast and/or speed. To address this challenge, we developed a novel confocal microscope that allows simultaneous multiplane imaging with high-contrast at near-kHz rates. This approach enables high signal-to-noise ratio voltage imaging in densely labeled populations and minimizes optical crosstalk during concurrent optogenetic photostimulation.

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Weber, T. D., Moya, M. V., Mertz, J., Economo, M. N.. 2021-12-13. High-speed, multi-Z confocal microscopy for voltage imaging in densely labeled neuronal populations. https://doi.org/10.1101/2021.12.10.472140

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