Microbiota induces aging-related leaky gut and inflammation by dampening mucin barriers and butyrate-FFAR2/3 signaling

Mishra, S. P.; Wang, B.; Wang, S.; Nagpal, R.; Miller, B.; Jain, S.; Lee, J. Y.; Borlongan, C.; Taraphder, S.; Rane, S. G.; Yadav, H.

doi:10.1101/2021.08.18.456856

bioRxiv · 10.1101/2021.08.18.456856

Microbiota induces aging-related leaky gut and inflammation by dampening mucin barriers and butyrate-FFAR2/3 signaling

Mishra, S. P.·Wang, B.·Wang, S.·Nagpal, R.·Miller, B.·Jain, S.·Lee, J. Y.·Borlongan, C.·Taraphder, S.·Rane, S. G.·Yadav, H.

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Abstract

Increased chronic inflammation is one of the key risk factors of aging-related disorders although its precise etiology remains elusive. Here, we demonstrate that aged, but not young, microbiota triggers inflammation by promoting gut permeability (leaky gut) via disruption of mucus barriers. Levels of the beneficial short-chain fatty acid, butyrate, are suppressed in the aged gut. Consistent with feedback regulation, the expression of butyrate-sensing receptors, free fatty acid receptor 2/3 (FFAR2/3), are also reduced in aged gut. Butyrate treatment of aged mice revereses the reduced mucin production, increased gut permeability and inflammation associated with low butyrate levels. In agreement, intestine-specific FFAR2/3 knockout mice manifest a compromised gut phenotype typically seen in aged mice,, such as increased gut permeability and inflammation with reduced mucin production. Taken together, our results demonstrate that an aged gut microbiota causally instigates inflammation by increasing gut permeability due to reduced butyrate levels, FFAR2/3 expression, and mucin barriers. Thus, butyrate-FFAR2/3 agonism could ameliorate the deleterious effects seen in aged gut and their implications on metabolic health.

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Mishra, S. P., Wang, B., Wang, S., Nagpal, R., Miller, B., Jain, S., Lee, J. Y., Borlongan, C., Taraphder, S., Rane, S. G., Yadav, H.. 2021-08-19. Microbiota induces aging-related leaky gut and inflammation by dampening mucin barriers and butyrate-FFAR2/3 signaling. https://doi.org/10.1101/2021.08.18.456856

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