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bioRxiv · 10.1101/2021.07.31.454512

Recombinant single-cycle influenza virus as a new tool to augment antitumour immunity with immune checkpoint inhibitors

Kandasamy, M.
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Abstract

Virus-based tumour vaccines offer many advantages compared to other antigen delivering systems. They generate concerted innate and adaptive immune response, and robust CD8+T cell responses. We engineered a non-replicating pseudotyped influenza virus (S-FLU) to deliver the well-known cancer testis antigen, NY-ESO-1 (S-NY-ESO-1 FLU). Intranasal or intramuscular immunization of NY-ESO-1 S-FLU virus in mice elicited a strong NY-ESO-1 specific CD8+T cell response in lungs and spleen that resulted in the regression of NY-ESO-1 expressing lung tumour and subcutaneous tumour respectively. Combined administration with anti PD-1 antibody, NY-ESO-1 S-FLU virus augmented the tumour protection by reducing the tumour metastasis. We propose that the antigen delivery through S-FLU is highly efficient in inducing antigen specific CD8+T cell response and protection against tumour development in combination with PD-1 blockade.

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Kandasamy, M.. 2021-08-01. Recombinant single-cycle influenza virus as a new tool to augment antitumour immunity with immune checkpoint inhibitors. https://doi.org/10.1101/2021.07.31.454512

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