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bioRxiv · 10.1101/2021.07.24.453620

Targeted drug delivery using Beeswax derived Albendazole-Loaded Solid Lipid Nanoparticles in Haemonchus contortus, an albendazole tolerant nematode.

Sharma, S.·Goel, V.·Kaur, P.·Gadhave, K.·Garg, N.·Singla, L. D.·Choudhury, D.
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Abstract

Targeted delivery has not been achieved for anthelmintic treatment, resulting in the requirement of excess drugs dose leading to side effects and therapeutic resistance. Gastrointestinal helminths ingest lipid droplets from digestive fluid for energy production, development, and defense. Worms habit inspired us to develop biocompatible, oral administrable, bee-wax derived solid lipid nanoparticles (SLN) with excellent drug (albendazole) loading efficiency of 83.3 {+/-} 6.5 mg/g and sustained-release properties (86.4 {+/-} 3.9 % of drug released within 24 h). Rhodamine B-loaded SLN showed time-dependent release and distribution of dye in vivo in Haemonchous contortus. The intestinal sustained-release property was shown by the particles that caused enhancement of albendazole potency for up to 50 folds. Therefore, this formulation has immense potential as an anthelminthic drug delivery vehicle that will not only be able to reduce the dose but will also reduce the drug-induced side effects by enhancing the bioavailability of the drug. HighlightsO_LIAlbendazole-loaded Solid Lipid Nanoparticles (SLN-A) were formulated using Beeswax as stating material showing high drug loading capacity of 83 mg/g with sustained-release properties and 84 {+/-} 3 % of drug release within 24 h. C_LIO_LISLN-A particles showed 50 fold enhancement of Albendazole activity against Haemonchous contortus worm. C_LIO_LIRhodamine B-loaded SLN particles showed the specific uptake and in-vivo sustained release of dye in the worm. C_LI

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Sharma, S., Goel, V., Kaur, P., Gadhave, K., Garg, N., Singla, L. D., Choudhury, D.. 2021-07-24. Targeted drug delivery using Beeswax derived Albendazole-Loaded Solid Lipid Nanoparticles in Haemonchus contortus, an albendazole tolerant nematode.. https://doi.org/10.1101/2021.07.24.453620

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