Major functional bias for mitochondrial complexes in genome-wide CRISPR screens

Rahman, M.; Billmann, M.; Costanzo, M.; Aregger, M.; Tong, A. H. Y.; Chan, K.; Ward, H. N.; Brown, K. R.; Andrews, B. J.; Boone, C.; Moffat, J.; Myers, C. L.

doi:10.1101/2020.08.31.273730

bioRxiv · 10.1101/2020.08.31.273730

Major functional bias for mitochondrial complexes in genome-wide CRISPR screens

Rahman, M.·Billmann, M.·Costanzo, M.·Aregger, M.·Tong, A. H. Y.·Chan, K.·Ward, H. N.·Brown, K. R.·Andrews, B. J.·Boone, C.·Moffat, J.·Myers, C. L.

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Abstract

We present FLEX (Functional evaluation of experimental perturbations), a pipeline that leverages several functional annotation resources to establish reference standards for benchmarking human genome-wide CRISPR screen data and methods for analyzing them. We apply FLEX to analyze data from the diverse cell line screens generated by the DepMap project. We identify a dominant mitochondria-associated signal, which our time-resolved CRISPR screens and analysis suggests may reflect screen dynamics and protein stability effects rather than genetic dependencies.

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Rahman, M., Billmann, M., Costanzo, M., Aregger, M., Tong, A. H. Y., Chan, K., Ward, H. N., Brown, K. R., Andrews, B. J., Boone, C., Moffat, J., Myers, C. L.. 2020-08-31. Major functional bias for mitochondrial complexes in genome-wide CRISPR screens. https://doi.org/10.1101/2020.08.31.273730

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