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bioRxiv · 10.1101/2020.08.11.244939

Diffusive dynamics of Aspartate α-decarboxylase (ADC) liganded with D-serine in aqueous solution

Raskar, T.·Niebling, S.·Devos, J. M.·Yorke, B. A.·Härtlein, M.·Huse, N.·Forsyth, T. V.·Seydel, T.·Pearson, A. R.
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Abstract

Incoherent neutron spectroscopy, in combination with dynamic light scattering was used to investigate the effect of ligand binding on the center-of-mass self-diffusion and internal diffusive dynamics of E.coli aspartate -decarboxylase (ADC). The X-ray crystal structure of the D-serine inhibitor complex with ADC was also determined, and molecular dynamics simulations used to further probe the structural rearrangements that occur as a result of ligand binding. These experiments reveal the existence of higher order oligomers of the ADC tetramer on ns-ms time-scales, and also show that ligand binding both affects the ADC internal diffusive dynamics and appears to further increase the size of the higher order oligomers.

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Raskar, T., Niebling, S., Devos, J. M., Yorke, B. A., Härtlein, M., Huse, N., Forsyth, T. V., Seydel, T., Pearson, A. R.. 2020-08-12. Diffusive dynamics of Aspartate α-decarboxylase (ADC) liganded with D-serine in aqueous solution. https://doi.org/10.1101/2020.08.11.244939

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