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bioRxiv · 10.1101/2020.04.30.069039

SARS-CoV-2 genomes recovered by long amplicon tiling multiplex approach using nanopore sequencing and applicable to other sequencing platforms

Resende, P. C.·Motta, F. C.·Roy, S.·Appolinario, L.·Fabri, A.·Xavier, J.·Harris, K.·Matos, A. R.·Caetano, B. C.·Garcia, C. C.·Miranda, M. D.·Ogrzewalska, M.·Abreu, A.·Williams, R.·Breuer, J.·Siqueira, M. M.
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Abstract

Genomic surveillance has become a useful tool for better understanding virus pathogenicity, origin and spread. Obtaining accurately assembled, complete viral genomes directly from clinical samples is still a challenging. Here, we describe three protocols using a unique primer set designed to recover long reads of SARS-CoV-2 directly from total RNA extracted from clinical samples. This protocol is useful, accessible and adaptable to laboratories with varying resources and access to distinct sequencing methods: Nanopore, Illumina and/or Sanger.

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Resende, P. C., Motta, F. C., Roy, S., Appolinario, L., Fabri, A., Xavier, J., Harris, K., Matos, A. R., Caetano, B. C., Garcia, C. C., Miranda, M. D., Ogrzewalska, M., Abreu, A., Williams, R., Breuer, J., Siqueira, M. M.. 2020-05-01. SARS-CoV-2 genomes recovered by long amplicon tiling multiplex approach using nanopore sequencing and applicable to other sequencing platforms. https://doi.org/10.1101/2020.04.30.069039

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